Impact of DNA Topology on cellular metabolism: in vivo and in vitro approaches to study chromatin dynamics and DNA Topoisomerases.
Ph.D. in Biology XXIX Cycle
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| Định dạng: | Luận án |
| Ngôn ngữ: | English |
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UNIVERSITA’ DEGLI STUDI DI NAPOLI FEDERICO II
2020
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| Truy cập trực tuyến: | http://thuvien.ued.udn.vn/handle/TVDHSPDN_123456789/56288 |
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oai:localhost:TVDHSPDN_123456789-562882022-10-14T07:53:07Z Impact of DNA Topology on cellular metabolism: in vivo and in vitro approaches to study chromatin dynamics and DNA Topoisomerases. Visone, Valeria Valenti, Anna DNA topology Cellular metabolism In vivo Chromatin dynamics Ph.D. in Biology XXIX Cycle DNA topology describes the tertiary conformations of DNA and it influences all the fundamental biological processes such as transcription, replication and recombination. DNA topoisomerases are essential enzymes able to covalently modify DNA topology and they are emerging as important factors in a wide range of fundamental metabolic processes in both the nuclear and mitochondrial genomes. Furthermore the study of topoisomerases is therapeutically relevant to cancer, immune disorders and neurological diseases. Topoisomerases introduce transient DNA breaks using a trans-esterification mechanism, which is highly reversible and minimizes the risks to genome stability that would otherwise occur owing to strand breakage. Furthermore the topological state of DNA is not equal among different classes of organisms. In mesophilic organisms DNA is negatively supercoiled while positive supercoiling is a peculiar mark of thermophilic organisms living at high temperatures. 1.Introduction 6 • 1.1 DNA Topology and Transcription 6 • 1.2 Structural diversity of DNA 7 • 1.3 Understanding Reverse Gyrase function 10 • 1.4 DNA Topoisomerases and their poisoning 11 References 14 2.Papers 20 • 2.1 Massively Systematic Transcript End Readout (MASTER): Transcription Start Site Selection, Transcriptional Slippage, and Transcript Yields 20 • 2.2 Comparison of Plasmid DNA Topology at nanoscale level 43 • 2.3 Heterologous expression of thermophilic reverse gyrase in an archaeal model system by exploiting a new thermostable protein tag 60 • 2.4 A new Daunomycin-Oligoarginine conjugate: biochemical characterization 70 • 2.5 Chromatin Structure and Dynamics in Hot Environments: Architectural Proteins and DNA Topoisomerases of Thermophilic Archaea 85 3.Conclusions and Future Perspectives 118 • 3.1 DNA Topology and Transcription 118 • 3.2 Structural diversity of DNA 119 • 3.3 Understanding Reverse Gyrase function 121 • 3.4 DNA Topoisomerases and their poisoning 123 References 2020-01-08T03:18:41Z 2020-01-08T03:18:41Z 2013 Thesis http://thuvien.ued.udn.vn/handle/TVDHSPDN_123456789/56288 en UNIVERSITA’ DEGLI STUDI DI NAPOLI FEDERICO II |
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Trường Đại học Sư phạm - Đại học Đà Nẵng |
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DSpace |
| language |
English |
| topic |
DNA topology Cellular metabolism In vivo Chromatin dynamics |
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DNA topology Cellular metabolism In vivo Chromatin dynamics Visone, Valeria Impact of DNA Topology on cellular metabolism: in vivo and in vitro approaches to study chromatin dynamics and DNA Topoisomerases. |
| description |
Ph.D. in Biology XXIX Cycle |
| author2 |
Valenti, Anna |
| author_facet |
Valenti, Anna Visone, Valeria |
| format |
Thesis |
| author |
Visone, Valeria |
| author_sort |
Visone, Valeria |
| title |
Impact of DNA Topology on cellular metabolism: in vivo and in vitro approaches to study chromatin dynamics and DNA Topoisomerases. |
| title_short |
Impact of DNA Topology on cellular metabolism: in vivo and in vitro approaches to study chromatin dynamics and DNA Topoisomerases. |
| title_full |
Impact of DNA Topology on cellular metabolism: in vivo and in vitro approaches to study chromatin dynamics and DNA Topoisomerases. |
| title_fullStr |
Impact of DNA Topology on cellular metabolism: in vivo and in vitro approaches to study chromatin dynamics and DNA Topoisomerases. |
| title_full_unstemmed |
Impact of DNA Topology on cellular metabolism: in vivo and in vitro approaches to study chromatin dynamics and DNA Topoisomerases. |
| title_sort |
impact of dna topology on cellular metabolism: in vivo and in vitro approaches to study chromatin dynamics and dna topoisomerases. |
| publisher |
UNIVERSITA’ DEGLI STUDI DI NAPOLI FEDERICO II |
| publishDate |
2020 |
| url |
http://thuvien.ued.udn.vn/handle/TVDHSPDN_123456789/56288 |
| work_keys_str_mv |
AT visonevaleria impactofdnatopologyoncellularmetabolisminvivoandinvitroapproachestostudychromatindynamicsanddnatopoisomerases |
| _version_ |
1768016140597985280 |